食品与发酵工业2011，Vol.37Issue(12)：54-57,4.
梨果棒曲霉素产生菌的分离及ITS区鉴定
Isolation of Main Patulin-producing Strains from Storage Pears and Their ITS Sequences Analysis and Identification
摘要
Abstract
This study isolated main patulin-producing strains from decayed Yang Xin and Lai Yang pears,analyzed and identified their morphological characteristics and ITS sequences.In this paper,patulin-producing strains were isolated from storage pears and test the concentration of patulin produced by HPLC.The fungal ITS region-specific primers were used to do ITS PCR amplification sequencing,through the blast analysis between ITS sequences and GenBank nucleic acid database,finally their biological classifications were identified and their relationships were analyzed.Result： 10 patulin-producing strains were isolated from storage pears,including 1 strains of Fusarium,2 strains of Aspergillus,and the remaining 7 strains of Penicillium.The phylogenetic tree showed that strain P-9 belonged to one group,the other nine strains to another group and they have different levels of the phylogenetic relationships.The main patulin-producing strains are Penicillium,and the P-1 has the strongest ability.关键词
梨果/棒曲霉素/棒曲霉素产生菌/高效液相色谱/ITSKey words
pear/patulin/patulin-producing strains/HPLC/ITS分类
轻工纺织引用本文复制引用
谢芳超,刘春霖,杨相政,陈义伦..梨果棒曲霉素产生菌的分离及ITS区鉴定[J].食品与发酵工业,2011,37(12):54-57,4.基金项目
公益性行业（农业）科研专项经费项目（nyhyzx07-026）资助 （）
