食品与机械2011，Vol.27Issue(5)：111-114,4.DOI:10.3969/j.issn.1003-5788.2011.05.028
LAMP法在阪崎肠杆菌快速检测中的应用
The application of loop mediated isothermal amplification(LAMP)technique for rapid detection of Enterobacter sakazakii
摘要
Abstract
Four primers including two inner primers, two outer primers were designed specifically to recognize the ompA gene of E. Sakazakii and a method of loop-mediated isothermal amplification was set up to detect Enterobacter sakazakii rapidly. The results suggested that the method had high speciality to 2 E. Sakazakii typical strains, 24 isolates and 18 non- E. Sakazakii . More over, it had good sensitivity with the lowest detectable limit of 8 CFU/mL. Through detecting the proficiency tests of E. Sakazakii in milk powder and 26 milk powder samples by this method, the results indicated that it had the same results as traditional biochemical method and may be used to detect samples.关键词
阪崎肠杆菌/ompA基因/环介导恒温扩增方法Key words
Enterobacter sakazakii/ ompA gene/ loop mediated isothermal amplification引用本文复制引用
张淑红,吴清平,徐晓可,张菊梅,郭伟鹏..LAMP法在阪崎肠杆菌快速检测中的应用[J].食品与机械,2011,27(5):111-114,4.基金项目
广东省科技计划项目(编号:2008A040101001 （）
2009B040500001) （）
广东省科学院青年科学研究基金项目(编号:qnjj20099) （编号:qnjj20099）
