沈阳农业大学学报2011，Vol.42Issue(4)：433-437,5.
辽宁地区辣椒疫病菌鉴定及同源性分析
Identification and Homology Analysis of the Pathogeny on Pepper Phytophthora Blight in Liaoning
摘要
Abstract
Identification and homology of the pathogeny were studied by the observation of morphology and sequence analysis of the internal transcribed spacer region of the ribosomal DNA (rDNA-ITS) on pepper Phytophthora blight in Liaoning. The primers of ITS4 and ITS5 were used to expand the twenty-five strains in polymerase chain reaction (PCR) method. The sequences were compared with the other known sequences of Phytophthora. The clustering analyses were carried out by Clustalx1.83 and MECA4.0 software. The strains colony was white, and the mycelium was flocculent, un-septum. The sporangium was single, ovoid or pyriform, and the mastoid was single. The sequences of ITS were 805-877bp. The homologies of rDNA-ITS sequences were very approximate. Twenty-five strains were identified as Phytophthora capsici Leonian. The genetic distances of twenty-five strains were very close and there were not obvious regional characters among the strains from different areas.关键词
辣椒疫病菌/形态学/ITS序列测定/同源性分析Key words
Phytophothora capsici/ morphology/ rDNA-ITS/ homology analysis分类
农业科技引用本文复制引用
王辉,刘长远,赵奎华,孙军德..辽宁地区辣椒疫病菌鉴定及同源性分析[J].沈阳农业大学学报,2011,42(4):433-437,5.基金项目
农业部公益性行业科研专项项目(201003004) （201003004）
辽宁省教育厅科研项目(L2010497) （L2010497）
辽宁省科技攻关项目(2010215003) （2010215003）
