沈阳农业大学学报2011，Vol.42Issue(4)：443-448,6.
pb2+/Cd2+处理对冬小麦幼苗根际微生物种群数量的影响
Effects of Pb2+/Cd2+ on the Number of Microorganisms in the Rhizosphere of Winter Wheat Seedlings
摘要
Abstract
Pot experiments were conducted to culture winter wheat seedlings. The rhizosphere soil was collected to determine the number of bacteria, fungi, actinoplanes, nitrogen-fixing bacterial, and nitrosobacterial when seedlings had grown for three weeks, seven weeks, and twelve weeks, respectively. The number of bacteria, nitrogen-fixing bacteria, and nitrosobacteria in Pb27Cd2+ significantly (p<0.05) decreased by 20.41 %-49.79%, 26.62%-76.99%, and 87.15%-95.88%, respectively, and the number of fungi and actinoplanes increased by 0.31%-38.88% and 19.58%-113.64%, respectively at the third week. Moreover, the total number of microorganisms decreased and the diversity and the ratio of fungi / bacteria increased in Pb2+/Cd2+ at the third week. When winter wheat seedlings grew for seven weeks, the number of actinoplanes, nitrogen-fixing bacteria, and nitrosobacterial mainly increased by 18.16%-19.72%, 13.71%-24.59%, and 2.09%-519.29%, respectively and that of fungi decreased by 9.59%-39.46%. Moreover, the number bacterial increased in low doses of Pb2+/Cd2+ and decreased in high doses of Pb2+/Cd2*. At the same time, the total number of microorganisms increased and the diversity and the ratio of fungi/bacteria decreased in the stress of Pb2+/Cd2+. When winter wheat seedlings grew for twelve weeks in Pb22+/Cd2*, the number of bacteria significantly (p<0.01) increased, and the number of fungi, actinoplanes, nitrosobacterial, and nitrogen-fixing bacterial decreased by 12.05%-44.66%, 1.94%-15.51%, 12.44%-55.72%, and 97.88%-99.00%, respectively. At the same time, the total of microorganism mainly increased, the diversity reduced, and the ratio of fungi / bacterial significantly (p<0.01) decreased. The effect of Pb2+/Cd2* on the number of rhizosphere microorganisms, diversities, and the ratio of fungi/bacteria was significant (p<0.05) and the shift of the diversity was mainly due to the change of the number of actinoplanes. The irregular change of nitrosobacterial and nitrogen-fixing bacteria indicated that the nitrogen fixed capacity of soil in the rhizosphere of winter wheat seedlings was affected by Pb2+/Cd2* in the future. Moreover, the utilization of nitrogen resource was also affected.关键词
Pb2+/Cd2+/冬小麦/根际微生物/多样性指数/真菌/细菌比值Key words
Pb2+/Cd2+/ winter wheat/ rhizosphere soil microbial/ diversity index/ ratio of fungi/bacterial分类
农业科技引用本文复制引用
贾夏,周春娟,董岁明..pb2+/Cd2+处理对冬小麦幼苗根际微生物种群数量的影响[J].沈阳农业大学学报,2011,42(4):443-448,6.基金项目
国家自然科学基金项目(31000222) （31000222）
中央高校基金项目(CHD2009JC144) （CHD2009JC144）
