沈阳农业大学学报2011，Vol.42Issue(4)：449-453,5.
牧羊犬IFN-β3基因的克隆和序列分析
Cloning and Sequence Analysis of Shepherd Dog IFN-β Gene
摘要
Abstract
The interferon-p (IFN-p) gene of shepherd dog was amplified by PCR from genome DNA of blood cell, then cloned into pGEM-T easy vector and transformed into the competent cell of E.coli JM109. The positive clone was sequenced after identification of restriction endonuclease cleavage and PCR amplification. Shepherd dog IFN-P consisted of 561 bp and coded for 186 amino acids of which the first 21 amino acids constituted a putative signal peptide. The mature protein with the predicted molecular weight of 21 kD and the isoelectric point of 5.737 contained 2 potential casein kinase II phosphorylation sites, 5 N-glycosylation sites and 4 cysteines, and most of which were hydrophilic region. The protein mainly composed of 5 α-helix domains according to the secondary and tertiary structure prediction. The IFN-(3 gene of shepherd dog was identity to that of red fox and shared high homology of 85 %-98.4 % with raccoon dog, ferret and domestic cat. They grouped into the same branche in the phylogenetic tree base on IFN-P gene.关键词
犬/干扰素-β(IFN-β)/基因克隆/序列分析Key words
canine (dog)/ interferon-P (IFN-P)/ gene cloning/ sequence analysis分类
农业科技引用本文复制引用
夏润玺,张曼夫..牧羊犬IFN-β3基因的克隆和序列分析[J].沈阳农业大学学报,2011,42(4):449-453,5.基金项目
沈阳农业大学青年教师科研基金资助项目(2006210) （2006210）
