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酶解法制备白藜芦醇的工艺优化

陈蓉蓉 姜华 蒲含林

中国食品添加剂Issue(5):64-67,4.
中国食品添加剂Issue(5):64-67,4.

酶解法制备白藜芦醇的工艺优化

Optimization technology for production of resveratrol by enzyme

陈蓉蓉 1姜华 1蒲含林1

作者信息

  • 1. 暨南大学生物工程研究所,广州510632
  • 折叠

摘要

Abstract

The optimization technology for extraction Resveratrol from Polygonum cuspidatum were studied. The effect of complex enzyme, cellulose, β - glucosidase and Polydatin special enzyme on the extraction was investigated. The experiment results show that the content of resveratrol by β - glucosidase was the highest. The single factor experiment design was used and resveratrol was choosen as the index to study the key factors affecting the enzymic hydrolysis, such as substrate concentration, enzyme dosage, pH, the enzymic hydrolysis temperature and the time. The optimum enzymic hydrolysis procedure was; pH was 5, the amount of the β - glucosidase was 3% , the temperature was 40℃ , and the enzymic hydrolysis reacting time was 10h. The technology improves the extracting rate and is suitable for mass production.

关键词

虎杖/β-葡萄糖苷酶/酶解/白藜芦醇

Key words

polygonum cuspidatum/ β - glucosidase/ enzymic hydrolysis/ resveratrol

分类

医药卫生

引用本文复制引用

陈蓉蓉,姜华,蒲含林..酶解法制备白藜芦醇的工艺优化[J].中国食品添加剂,2011,(5):64-67,4.

中国食品添加剂

OA北大核心CSTPCD

1006-2513

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