中国食品添加剂Issue(6)：109-112,4.
竹叶中总黄酮的纯化工艺研究
Study of purification technique of flavones from bamboo leaves
摘要
Abstract
To optimize the purification technique of flavones from bamboo leaves by comparing the different extraction with different purification techniques. Methods: The content of flavones was determined by ultraviolet spectrophotometry and the content of isoorientoside was determined by HPLC. Acetidin extraction method and resin column separation method were compared. Then the purification technique was optimized. Results: The polycaprolactam column absorption method was the best. The purification condition was: defated extracting solution was mixed with resin. Impurity was eluted by water and then by 50% ethanol at 9BV. The content of flavones was 35%. Conclusion: The polycaprolactam column absorption method used in purification technique of flavones from bamboo leaves is simple with high yield and purity. It has no pollution and can be used in large - scale production.关键词
竹叶/黄酮/异荭草苷/紫外分光光度法/高效液相色谱法/聚酰胺柱分离法Key words
bamboo leaves/flavones/isoorientoside/ultraviolet spectrophotometry/HPLC/polycaprolactam column absorption method分类
轻工纺织引用本文复制引用
雷文惠,刘强,刘莉..竹叶中总黄酮的纯化工艺研究[J].中国食品添加剂,2011,(6):109-112,4.基金项目
广东省自然科学基金项目(8451051501000166). （8451051501000166）
