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响应面法优化低乳糖奶水解工艺

徐晨 杜鉴 陈历俊 石维忱

中国食品添加剂Issue(6):210-214,5.
中国食品添加剂Issue(6):210-214,5.

响应面法优化低乳糖奶水解工艺

Optimization of low- lactose milk manufacture using response surface methodology

徐晨 1杜鉴 2陈历俊 3石维忱2

作者信息

  • 1. 大连工业大学,大连116034
  • 2. 北京三元食品股份有限公司,北京100085
  • 3. 中国食品添加剂和配料协会,北京100020
  • 折叠

摘要

Abstract

Nutrient-rich milk and dairy products, easy digestion and absorption, known as "white blood" , is the best natural food. China dairy industry has developed rapidly in recent years, but there is still a huge gap in average level compared to the world. And lactose intolerance is one of the most important reason for restricting the development of China dairy industry. Using β-galactosidase hydrolysis of milk can generate glucose, galactose and the " bifidus factor" galactooligosaccharide, which Not only solve the problem of lactose intolerance, but also increase the nutritional value of milk. On the basis of single factor experiment, the best hydrolysis technique parameter were acquired by response analysis in which the galactooligosaccharide synthesis rate was the main indicator. The results showed that when the reaction temperature was 45. 3 ℃ , enzyme concentration was 2. 5ml and reaction time was 69. 7min, the maximum galactooligosaccharide yield was 6. 15%. The lactulose content in low-lactose milk was 158. 17mg/L determined by lactulose kit.

关键词

低聚半乳糖/β-半乳糖苷酶/响应面法/低乳糖奶

Key words

galactooligosaccharide/p-galactosidase/response surface methodology/low-lactose milk

分类

轻工纺织

引用本文复制引用

徐晨,杜鉴,陈历俊,石维忱..响应面法优化低乳糖奶水解工艺[J].中国食品添加剂,2011,(6):210-214,5.

基金项目

北京市科技计划(D10110504600000). (D10110504600000)

中国食品添加剂

OA北大核心CSTPCD

1006-2513

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