中国食品添加剂Issue(6)：218-221,4.
高效液相色谱法快速测定烹炸油中苯并(a)芘的研究
Fast determination of Benzo (a) pyrene in fried oil by high performance liquid chromatography
张旭 1郑睿行 1鲍利锋1
作者信息
- 1. 衢州市质量技术监督检测中心,衢州324002
- 折叠
摘要
Abstract
benzo (a)pyrene is a common high activity indirect carcinogens, benzo (a)pyrene through a food processing and other way into food, High - performance liquid chromatography ( HPLC) was used for determination of benzo (a) pyrene in fried oil. C18 as the separation column. The mobile phase of acetonitrile; water, excitation wavelength of fluorescence detector 384nm and detection wavelength 406 nm. Temperature of column 30℃ , and the injection volume of 10μl. The calibration curve is linear in the range of 1 ~40μg/L, Y = 1. 5758X +0. 05705, R2 = 0. 9998, the limit of detection was 0. Μg/kg, recoveries were more than 95% , and relative standard deviation (RSD)was no more than 2. 5%. The method has advantages of an accuracy and simple process of sample treatment, short analysis time, is suitable for qualitative and quantitative analysis of benzo (a)pyrene in fried oil, and is a good detection method of benzo (a)pyrene in fried oil.关键词
苯并(a)芘/高效液相色谱法/烹炸油Key words
benzo (a)pyrene/high Performance liquid chromatography/fired oil分类
轻工纺织引用本文复制引用
张旭,郑睿行,鲍利锋..高效液相色谱法快速测定烹炸油中苯并(a)芘的研究[J].中国食品添加剂,2011,(6):218-221,4.
