中国食品学报2011，Vol.11Issue(8)：87-94,8.
复合酶解带鱼蛋白制备亚铁螯合多肽的工艺优化
Optimization of Composite Enzymatic Hydrolysis Technology for Preparation of Ferrous Chelating Polypeptide from Hairtail Protein
摘要
Abstract
In this study, waste hairtail protein was used as the raw material to prepare polypeptides by enzymatic hydrolysis. In order to maximize degree of hydrolysis (DH) and ferrous chelating rate, the optimal hydrolysis mode of hairtail was selected from hydrolysis with pairwise combination of alkaline protease and papaya protease that resulted in higher degree of hydrolysis and ferrous chelating rate than neutral protease, trypsin and pepsin, or each of them.Mean-while, based on this, the optimal values of crucial technological parameters, including temperature, hydrolysis time, pH and total enzyme dose, were determined by the method of quadratic regression orthogonal rotation combination design. Results showed that the optimal enzymatic production process was based on sequential hydrolysis at 45 ℃ with alkaline protease for 9 h at pH 8.0 followed by papaya protease for8 h at pH 6.0 using a mixture of alkaline protease and papaya protease at 4:6 activity ratio with 22 000 U/g total enzyme dose, which gave 52.22% DH and 82.31% ferrous chelating rate.关键词
带鱼蛋白/复合酶解/亚铁螯合多肽/二次回归正交旋转组合设计Key words
hairtail protein/composite enzymatic hydrolysis/ferrous chelating polypeptide/quadratic regression orthogonal rotation combination design引用本文复制引用
林慧敏,邓尚贵,庞杰,陈丹洁,马鹏飞..复合酶解带鱼蛋白制备亚铁螯合多肽的工艺优化[J].中国食品学报,2011,11(8):87-94,8.基金项目
国家自然科学基金资助项目(No.31071628) （No.31071628）
