中国食物与营养2011，Vol.17Issue(9)：24-28,5.
萜类合成甲羟戊酸途径的关键基因的克隆及表达分析
Cloning and Expression Analysis of Key Genes in MVA Pathway
汪婉宜 1申业 2黄璐琦 2陈平1
作者信息
- 1. 武汉工业学院,武汉430001
- 2. 中国中医科学院中药研究所,北京100700
- 折叠
摘要
Abstract
【Objective】 To clone the gene length of the transcription factors in Salvia miltiorrhiza and analyze its initial expression and induced expression in Salvia miltiorrhiza.【Method】 Guided by high-throughput sequencing results and inspired from BHLH genes reported in arabidopsis and rice,full-length gene was cloned.We studied this particular BHLH gene,and analyzed intial expression and the induced expression on each part of Salvia miltiorrhiza.A 500bp sequence of SMbHLH-2 was cloned from the gen-omic DNA of Salvia miltiorrhiza.Through the ABA,MJ,AG ＋ and other biotic and abiotic inducible factor induction,we detected and analyzed the expression of the roots＇ during different induction time.【Result】 We found that increasing transcriptional activity of transcription factors might increase its transcription efficiency,which might increase the amount of secondary metabolites,thus improve the plant＇s medicinal active ingredient production.关键词
甲羟戊酸（MVA）途径/cDNA构建/SMBHLH转录因子/诱导子/丹参Key words
MVA pathway/cDNA construction/SMBHLH transcription factor/Salvia miltiorrhiza分类
农业科技引用本文复制引用
汪婉宜,申业,黄璐琦,陈平..萜类合成甲羟戊酸途径的关键基因的克隆及表达分析[J].中国食物与营养,2011,17(9):24-28,5.
