中国食物与营养2011，Vol.17Issue(9)：74-76,3.
Bcl-xl和p53基因在条斑紫菜多糖抑制胃癌BGC-823细胞增殖中的作用
Effect of Bcl-xl and p53 Gene on Porphyra yezoensis Polysaccharide in Inhibitory Cell Proliferation in Gastric Carcinoma BGC-823 Cells
摘要
Abstract
【Objective】To investigate the effect of Bcl-xl and p53 gene on BGC-823 cells proliferation and apoptosis induced by Porphyra yezoensis polysaccharide.【Method】 BGC-823 cells were treated with various of Porphyra yezoensis polysaccharide.We detected the rate of cell proliferation inhibition by MTT assay and the levels of Bcl-xl and p53 gene expression by RT-PCR.We detected cell cycle and cell apoptosis by flow cytometry.【Result】Porphyra yezoensis polysaccharide could effectively inhibit the BGC-823 cells proliferation and induce apoptosis,and it could effectively decrease the level of Bcl-xl gene expression and recrease the level of p53 gene expression.【Conclusion】Porphyra yezoensis polysaccharide could inhibite the BGC-823 cells proliferation and induce apoptosis of BGC-823 cells probably through down regulating Bcl-xl gene expression and up regulating p53 gene expression.关键词
条斑紫菜多糖/BGC-823细胞/Bcl-xl基因/p53基因Key words
Porphyra yezoensis polysaccharide/BGC-823 cells/Bcl-xl gene/p53 gene分类
生物科学引用本文复制引用
张杰,杨旭东,申梅淑..Bcl-xl和p53基因在条斑紫菜多糖抑制胃癌BGC-823细胞增殖中的作用[J].中国食物与营养,2011,17(9):74-76,3.
