高效液相色谱法同时测定双黄消炎片中黄芩苷和黄芩素含量OACSTPCD
Simultaneous Determination of Baicalin and Baicalein in Shanghuangxiaoyan Tablets by HPLC
目的 建立同时测定双黄消炎片中黄芩苷和黄芩素含量的反相高效液相色谱法.方法 色谱柱采用Agilent Eclipse XDB-C18柱(250 mm ×4.6 mm,5μm),以甲醇-0.1%磷酸溶液为流动相(梯度洗脱),检测波长为277 nm,流速1.0 mL/min,进样量10μL,柱温35℃.结果 黄芩苷质量浓度在5.678~141.95μg/mL范围内与峰面积有良好线性关系,r=1.000 0(n=7);黄芩素质量浓度在2.122~53.…查看全部>>
Objective To establish RP - HPLC method for simultaneous determination of Baicalin and Baicalein In shuanghuangxiaoyan tablets. Methods Using a column packed with Agilent Eclipse XDB - C18( 250 mm×4. 6 mm,5 μm ). The mobile phase was methanol-D. 1%H3PO4 by gradient elution and the detection wavelength was 277 nm,the flow rate was 1. 0 mL/min,the injection volume was 10(J.L,the column temperature was 35 ℃. Results The linear response of baicalin range f…查看全部>>
徐占方;陈立柱;姜雪敏;孙菲
黑龙江省鸡西市药品检验所,黑龙江鸡西,158100黑龙江省鸡西市药品检验所,黑龙江鸡西,158100黑龙江省鸡西市药品检验所,黑龙江鸡西,158100黑龙江省鸡西市药品检验所,黑龙江鸡西,158100
医药卫生
含量测定黄芩苷黄芩素高效液相色谱法
content determinationbaicalinbaicaleinHPLC
《中国药业》 2012 (4)
28-29,2
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