现代食品科技2012，Vol.28Issue(3)：270-273,4.
巴氏杜氏藻八氢番茄红素合成酶侧翼调控序列的克隆与分析
In silico Analysis of Phytoene Synthase and its Promoter Reveals Hints for Regulation Mechanisms of Carotenogenesis in Duanliella bardawil
唐春晖 1劳永民 1姜建国1
作者信息
- 1. 华南理工大学生物科学与工程学院,广东广州510006
- 折叠
摘要
Abstract
Previous researches showed that phytoene synthase (PS Y) is the first regulatory point in carotenogenesis in Dunaliella banhwil. In this study, two sets of adjacent gene specific primers (pSPl, pSP2 and pSP3; tSPl, tSP2 and tSP3) were designed and LA PCR-based genomic walking approach was performed for isolation of psy promoter and terminator in D.bardawil, respectively; and subsequent analysis of conserved regulatory sequences were conducted by online promoter analysis softwares. Finally, using bioinformatics methods, the protein structure of DbPSY was also predicted. The calculated results found two conserved regulatory sequences in PSY promoter: BOXLCOREDCPAL and GT1GMSCAM4. BOXLCOREDCPAL was induced by UV to up-regulate expression of downstream gene; and GT1GMSCAM4 up-regulates expression of downstream gene induced by NaCl. Protein structure analysis demonstrated low homology of N-terminus between D. Bardawil and other algae. Therefore we speculated the regulation mechanisms may partly involve in the conserved sequences of psy promoter and the diversity of N-terminus of DbPSY compared to other species.关键词
八氢番茄红素合成酶/巴氏杜氏藻/基因组步移/启动子/保守调控序列Key words
phytoene synthase/ Dunaliella bardawil/ genomic walking/ promoter/ conserved regulatory sequence引用本文复制引用
唐春晖,劳永民,姜建国..巴氏杜氏藻八氢番茄红素合成酶侧翼调控序列的克隆与分析[J].现代食品科技,2012,28(3):270-273,4.
