食品与机械2012，Vol.28Issue(1)：78-81,4.DOI:10.3969/j.issn.1003-5788.2012.01.020
玉米赤霉烯酮多克隆抗体的制备及特性分析
Preparation and characterization of polyclonal antibodies against zearalenone
摘要
Abstract
The hapten of zearalenone with carboxyl group was synthe-tizedt and then identified using liquid chromatography/mass spec-trometry. The hapten was coupled with carrier protein ovabumin (OVA) or bovine serum albumin(BSA) to prepare artificial antigen by active ester method. And then, was identified by SDS-PAGE and UV scanning spectrum. Three mice were immunized to prepare poly-clonal antibody of zearalenone . Titres of antibodies were determined to be 1 : 32 000 by indirect ELISA, and the antibodies showed high sensitivity with Icso value of 39. 8 ng/mL toward zearelanone. Cross-reactivities with analogues were as follows: β-zearalenol, 4. 8%; zearalanone,3. 07% ; crzearalanoU 0. 96%; 0-zearalanol, 0. 09%;The results show that the artifical antigens and polyclonal antibody of zearalenone were developed successfully.关键词
玉米赤霉烯酮/F-2毒素/活泼酯法/人工抗原/酶联免疫吸附试验/多克隆抗体Key words
zearalenone/ F-2 toxin/ active ester method/artificial antigen/ ELISA/ polyclonal antibody引用本文复制引用
余涛,李大刚,邓宁,向军俭,宋其芳,王宏..玉米赤霉烯酮多克隆抗体的制备及特性分析[J].食品与机械,2012,28(1):78-81,4.基金项目
天河区科技计划项目(编号:108ZH044) （编号:108ZH044）
暨南大学创新基金项目(编号:11610312) （编号:11610312）
