沈阳农业大学学报2011，Vol.42Issue(5)：549-554,6.
光暗不同培养条件下植物生长调节剂对亚洲百合鳞茎诱导的影响
Lilium Asiatic Hybrid ‘Elite' Bulblets Formation in Vitro: Effects of Plant Growth Regulators as Well as Culture Condition
摘要
Abstract
Scales of Lilium Asiatic hybrid 'Elite' were used to study the effects of BA, NAA, sucrose, activated charcoal, salicylic acid and paclobutrazol on bulblets inducement in light or darkness culture. The results showed there were two bulblets inducement pathways. First, the bulblets were induced directly from scales. Second, buds formed in light culture and inflated into bulblets after 40 days. The multiplication of bulblets in light was higher than in dark culture. However, the diameter of bulblets induced in light was smaller than the dark. Sucrose, AC or paclobutrazol all could expand the bulblets. AC could also improve the bulblet quality. When 0.5g ? L-1 AC was added to the medium, the cohesion of bulblets scale was more tightly and closely. SA improved the proliferation of bulblets. 0.5 μmol-L-1 SA could increase the rate of bulblets formation, reaching to 96% .The best medium for the bulblets formation and growth in vitro was MS+0.5mg ? L-1 BA+0.6mg-L-1 NAA+0.5g-L-1 AC+0.5u,mol·L-1 SA+ 5mg-L-1 Paclobutrazol+70g ? L-1 sucrose.关键词
亚洲百合/试管鳞茎/植物生长调节剂/光培养/暗培养Key words
Lilium Asiatic hybrid/ bulblet/ growth regulator/ light culture/ darkness culture分类
农业科技引用本文复制引用
孙红梅,张静,李雪艳,王寅寅,王春夏,王锦霞..光暗不同培养条件下植物生长调节剂对亚洲百合鳞茎诱导的影响[J].沈阳农业大学学报,2011,42(5):549-554,6.基金项目
国家自然科学基金项目(30972023) （30972023）
中国博士后科学基金项目(20090451280) （20090451280）
辽宁省重点实验室项目(LS2010148) （LS2010148）
辽宁省农业攻关项目(2010215003) （2010215003）
