沈阳农业大学学报2011，Vol.42Issue(5)：570-573,4.
风信子DNA不同提取方法的效果比较
Comparison of Genomic DNA Extraction Methods From Hyacinth
摘要
Abstract
Five methods including simple CTAB, CTAB improved method, SDS-CTAB method, high salt precipitation method and silica-purification method were used to extract genomic DNA from different parts of Hyacinth such as bud, leaves, scale. The results were compared by three parts, purity, the electrophoreses results and yield of the extracted DNA. The results showed that all of these DNA extraction methods except for CTAB-silica-purification method could obtain DNA. The purity of extracted DNA was as following : simple CTAB method >SDS-CTAB method >CTAB improved method>high salt precipitation method, and yield as following : simple CTAB method >CTAB improved method >high salt precipitation method >SDS-CTAB method. Simple CTAB method could obtain more purity DNA than the other methods and OD260/OD280 was 2.01,OD260/OD230 was 2.33. The concentration was 406.8ng-L~1 and the yield was 175.95ng·g-1.The extraction results from different parts showed that flower buds and leaves were both suit for Hyacinth obtain DNA.关键词
风信子/基因组DNA/提取方法Key words
Hyacinthus/ genomic DNA/ extraction methods分类
农业科技引用本文复制引用
胡凤荣,任翠,鲍仁蕾,罗凤霞..风信子DNA不同提取方法的效果比较[J].沈阳农业大学学报,2011,42(5):570-573,4.基金项目
国家林业局“948”项目(2009-4-12) （2009-4-12）
中国博士后科学基金面上项目(20100470217) （20100470217）
江苏省高校优势学科建设工程资助项目 （）
