中国食品学报2012，Vol.12Issue(2)：124-130,7.
基于多壁碳纳米管/SDBS/硫堇阪崎肠杆菌免疫传感器的研究
Studies on Immunosensor for Enterobacter Sakazakii Based on Multi-Wall Carbon Nanotubes/SDBS and Thionine
摘要
Abstract
Object: For the purpose of establishing the accurate electrochemical method for detecting Enterobacter sakazakii (E. Sakazakii), a sensitive and stable immunosensor was successfully fabricated. Method: First, multi-wall carbon nanotube (MWCNT)/sodium dodecylbenzene sulfonate (SDBS) homogeneous composite was dropped on the surface of the 4-channel screen-printed carbon electrode. Then Thionine was chemisorbed by carboxylic MWCNT. Furthermore, horseradish peroxidase labeled antibody to E. Sakazakii (HRP -anti-E.sakazakii) was chemisorbed onto Thionine film through the electrostatic force with the amino group of Thionine. The preparation process of modified electrode was characterized with atomic force microscope (AFM). Cyclic voltammetry (CV) was carried out to characterize the electrochemical properties of different electrodes and detect E. Sakazakii. Results: under optimal conditions, concentration of E. Sakazakii from 102~108 cfu/mL could be detected with a detection limit of 5.7×101cfu/mL (S/N=3). Conclusions: The enzyme immunosensor exhibited high specificity, satisfactory reproducibility (RSD=6.3%), stability (electrical response was 93.24% of initial value after storage in sterile container at 4 ℃ for 15 d) and accuracy (coincidence rate was 96.67% with GB/T 4789.40-2010). The novel immunosensor had potential application for rapid detection.关键词
免疫传感器/多壁碳纳米管/硫堇/阪崎肠杆菌/快速检测Key words
immunosensor/multi-wall carbon nanotube/Thionine/Enterobacter sakazakii/rapid detection引用本文复制引用
张晓,窦文超,赵广英..基于多壁碳纳米管/SDBS/硫堇阪崎肠杆菌免疫传感器的研究[J].中国食品学报,2012,12(2):124-130,7.基金项目
国家自然科学基金资助项目(No.30571623) （No.30571623）
浙江省重中之重项目(No.Z05-178) （No.Z05-178）
