中国食品学报2012，Vol.12Issue(2)：131-136,6.
抗Cry1c蛋白兔单克隆抗体的制备和鉴定
Preparation and Identification of Anti-Cry1c Protein Rabbit Monoclonal Antibodies
摘要
Abstract
Crylc protein was expressed by prokaryotic plasm id and after purification progress, Crylc protein was injected into rabbits as an antigen. After serum titer detection, cells fusion, hybridoma cell screening, plasmid DNA recombination, anti-Crylc monoclonal antibodies(MeAb) were obtained. After detecting some relation characters by ELISA, the results showed that the titer of McAb surpass 3.2×104, the affinity constant was about 2.4x10', there was little cross-reaction between CrylAc and anti-Crylc McAb, and no obvious cross-reaction between CrylAb and anti-Crylc McAb. The embedding amount of Cryle proteic was 400 ng/mL detected by indirect ELISA. It may be decide as positive.关键词
单克隆抗体/Cry1c/间接ELISA/蛋白/检测限Key words
monoclonal antibodies/Crylc/indirect ELISA/protein/detection limit引用本文复制引用
倪庚,沈金儿,热米拉,刘娜,冯劲松,郑晓冬..抗Cry1c蛋白兔单克隆抗体的制备和鉴定[J].中国食品学报,2012,12(2):131-136,6.基金项目
科技部转基因生物新品种培育科技重大专项(2009ZX08012-010B) （2009ZX08012-010B）
