茶叶科学2012，Vol.32Issue(2)：179-188,10.
“紫娟”晒青绿茶色素的HPLC-ESI-MS分离鉴定及其稳定性研究
HPLC-ESI-MS Analysis of Pigments in Zijuan Green Tea and Its Stability
摘要
Abstract
The pigments and its stability in Zijuan sun-dried green tea were investigated in this study. The pigments from Zijuan sun-dried green tea were extracted by acidic methanol-H2O solution and H2O, and the main compounds were identified by means of HPLC-ESI-MS/MS techniques. 14 anthocyanin, 24 flavonols, 8 phenolics and theaflavins and 3 alkaloids were identified from the extracts of Zijuan sun-dried green tea. The main anthocyanins, such as Cyanidin-3-glucoside, Cyaniding-3-galactoside, Cyanidin-3-0-(6-p-coumaroyl)-glucoside, Delphinidin-3-glucoside, Delphinidin-3-rutinoside, Delphinidin-3-0-(6-p-coumaroyl)-glucoside, Prodelphinidin B-2(or 4)3'-0-gallate, Procyanidin B-2(or 4)3'-O-gallate, Pefunidin-3-glc-4-vinylphenol and Malvidin-3-glc-4- vinylepicatechin etc were identified in Zijuan sun-dried green tea. The pigment extracted from Zijuan sun-dried green tea by using acidic methanol-methanol-HiO as solvent showed good water-solubility and was stable to light and heat in acid environment, but sensitive to the pH value of environment, oxidizer and reducer. The maximum adsorptionwavelength for the pigment is 538nm under pH 1.0. Fe2+ and Cu2+ can change the color of the pigments and stabilitywas decreased. The substance of the purple colour in Zijuan sun-dried green tea was clarified by HPLC-ESI-MS/MSanalysis.关键词
"紫娟"晒青绿茶/花色苷/黄酮类/多酚类/液相色谱-电喷雾质谱联用/稳定性Key words
Zijuan sun-dried green tea/ anthocyanins/ flavonoids/ phenolics/ HPLC-ESI-MS/ stability分类
轻工纺织引用本文复制引用
龚加顺,隋华嵩,彭春秀,范建功,李亚莉..“紫娟”晒青绿茶色素的HPLC-ESI-MS分离鉴定及其稳定性研究[J].茶叶科学,2012,32(2):179-188,10.基金项目
国家自然科学基金资助项目(No.30960241,30760152) （No.30960241,30760152）
