乳业科学与技术2011，Vol.34Issue(6)：272-274,3.
乳和乳制品中动物水解蛋白鉴定——比色法测定L（-）-羟脯氨酸含量
A Method for Identification of Hydrolyzed Animal Protein in Milk and Milk Products by Colorimetric Determination of L（-）-Hydroxyproline Content
王常捷 1仓义鹏 2王峰 1卢剑1
作者信息
- 1. 江苏省产品质量监督检验研究院,江苏南京210029
- 2. 宿迁市产品质量监督检验所,江苏宿迁223800
- 折叠
摘要
Abstract
Objective： To establish a colorimetric method to determine L（-）-hroxyproline content for identifying whether hydrolyzed animal protein is present in milk and milk products.Methods： L（-）-Hroxyproline was released from samples treated by acid hydrolysis and oxidized by chloramine T to form oxides containing pyrrole rings.Perchloric acid was then added to damage excess chloramine T.The L（-）-hroxyproline oxides were allowed to react with p-dimethylaminobenzaldehyde to generate red compounds for colorimetric determination at 558 nm wavelength.Results： Hydrolyzed animal protein was completely hydrolyzed into L（-）-hroxyproline after 6 h of hydrolysis at 110 ℃.The stability,reproducibility and spike recovery of the developed method were all consistent with requirements.关键词
动物水解蛋白/L（-）-羟脯氨酸/水解/比色Key words
hydrolysed animal protein/L（-）-hydroxyproline/hydrolysis/colorimetry分类
轻工纺织引用本文复制引用
王常捷,仓义鹏,王峰,卢剑..乳和乳制品中动物水解蛋白鉴定——比色法测定L（-）-羟脯氨酸含量[J].乳业科学与技术,2011,34(6):272-274,3.
