食品工业科技2012,Vol.33Issue(5):174-176,184,4.
紫外线和硫酸二乙酯诱变高产凝乳酶地衣芽孢杆菌的研究
Study on mutation breeding of Bacillus licheniformis producing chymosin by ultra violet and diethyl sulfate
摘要
Abstract
Bacillus licheniformis producing chymosin was mutated by ultraviolet and diethyl sulfate to improve the ability of producing chymosin.After screening repeatedly,a mutant DES-6 with high activity of chymosin and lower proteolysis was obtained.Compared with the original strain,the activity of chymosin increased by 15.41%,the proteolytic activity reduced by 64.80%.DES-6 had good hereditary stability after several generations.关键词
凝乳酶/地衣芽孢杆菌/诱变Key words
chymosin/Bacillus licheniformis/mutation分类
轻工纺织引用本文复制引用
张卫兵,梁琪,乔海军,米兰,张炎,杨敏,甘伯中..紫外线和硫酸二乙酯诱变高产凝乳酶地衣芽孢杆菌的研究[J].食品工业科技,2012,33(5):174-176,184,4.基金项目
国家863计划 ()
甘肃省科技重大专项 ()
甘肃农业大学校创新基金 ()