食品工业科技2012，Vol.33Issue(5)：230-234,5.
野生粉叶爬山虎种子等部位的原花青素提取纯化工艺及含量测定
Extraction technique conditions of procyanidins from seed and other parts of wild grown Parthenocissus thomsonili planch and its content determination
摘要
Abstract
Orthogonal experiments were designed for optimization of the extraction and purification technologic conditions of procyanidins from Parthenocissus thomsonili planch.Results showed that extraction for 80min,ethanol volume fraction of 75%,the optimum combined conditions were extraction temperature at 65℃,solution ratio of 1：10.After preliminary purification of extract solution,color reactions produced by vanillin hydrochloric acid method,the average procyanidins contents of stem,fruit peel and seed determined by ultraviolet spectrophotometry method were 2.6317%,3.7967%,4.0934% respectively.The results of isolation and purification of procyanidins by macroporous absorptive resin showed that LSA-21 was suitable for isolation and purification of procyanidins from Parthenocissus thomsonili planch,the optimum conditions of column isolation were that the mass concentration of initial feed solution was 20mg/mL,eluting speed was 2.0BV/h.The average contents of purified procyanidins of fruit peel,seed and stem determined by high performance liquid chromatography（HPLC） and ultraviolet spectrophotometry were 2.39%,2.31%,3.94% respectively.The purities of procyanidins purified by LSA-21 and determined by HPLC reached above 87%.关键词
粉叶爬山虎/原花青素/提取分离/HPLCKey words
Parthenocissus thomsonili planch/procyanidins/isolation and extraction/HPLC分类
轻工纺织引用本文复制引用
董爱文,朱深海,王国庆..野生粉叶爬山虎种子等部位的原花青素提取纯化工艺及含量测定[J].食品工业科技,2012,33(5):230-234,5.基金项目
湖南省科技厅资助项目 （）
湖南省科技厅课题 （）
张家界市科技局课题 （）
