食品工业科技2012，Vol.33Issue(6)：60-63,4.
改良的Folin-Ciocalteu比色法测定核桃外果皮中总多黔含量
Determination of total polyphenol in walnut husk with improved Folin- Ciocalteu colorimetry
摘要
Abstract
Objective：To set up an assay method for the determination of total polyphenol in walnut husk.Method：The determinatiolq conditions of total polyphenol content in walnut husk including the detection wavelength,the amount of sodium carbonate solution, Folin-Ciocalteu reagent, reaction temperature and time were studied and gallic acid was used as a standard.Results.The standard curve was linear in the range of 1-7~g/mL（y =0.1031x ＋ 0.0793, R2 = 0.9996） and the optimum conditions for determining total polyphenol in walnut husk were as followed：l.0mL of the walnut husk extract polyphenol, ].OmL of Folin-Ciocalteu reagent dosage and 4.0mL of 15% sodium carbonate solution,treatment at 30~C for 1.5h,the average recovery was 100.2% （RSD =0.91% ）.Under these conditions,the total polyphenol-content of walnut husk was 3.306mg /g.Conclusion：This method was simple and quick,the result was a~Jratp, r~.r~eatahl~, and .~tahl~..It can be used for the determination of total oolvohenol content in walnut husk.关键词
核桃外果皮/总多酚/含量测定/比色条件Key words
walnut husk/total polyphenol/determining content/colorimetric conditions分类
轻工纺织引用本文复制引用
徐佳,辛立方,张瑞廷,王华斌,傅力..改良的Folin-Ciocalteu比色法测定核桃外果皮中总多黔含量[J].食品工业科技,2012,33(6):60-63,4.基金项目
国家“十一五”科技支撑计划项目 （）
新疆维吾尔自治区高效科研计划科学研究重点项目（XJEDU2008113）. （）
