食品工业科技2012，Vol.33Issue(6)：91-94,4.
实时荧光定量RT-PCR监测沙门氏菌活菌
Real-time quantitative reverse transcription polymerase chain reaction detection of live Salmonella
摘要
Abstract
Salmonella was the most common pathogens that cause food poisoning.The conventional DNA- based PCR （DNA-PCR）detection method was rapid and sensitive, but dead bacteria could not be distinguished from the viable in the detection method, and the death may cause false-positive results.That could seriously affect the daily test results.Total RNA was extracted from Salmonella, by applying one-step reverse transcription polymerase chain reaction（RT-PCR）, to detect Salmonella invA mRNA, only live Salmonella was found positive and death was negative.And the RT-PCR had good stability, it could quickly and accurately quantify the live Salmonella, the detection limit was ]CFU/3mL in pure culture.Experiments showed that the RT-PCR was a fast, accurate method to detect live Salmonella.关键词
沙门氏茵/RT-PCR/死活茵/常规PCRKey words
Salmonella/RT- PCR/dead and live bacteria/conventional PCR分类
轻工纺织引用本文复制引用
张冲,刘祥,陈计峦..实时荧光定量RT-PCR监测沙门氏菌活菌[J].食品工业科技,2012,33(6):91-94,4.基金项目
国家质检总局科技项目（2010QK307）,天津市质监局科技项目（10-11）. （）
