食品工业科技2012，Vol.33Issue(6)：131-134,4.
胡桃醌抑制细菌生长作用的研究
Study on the effect of juglone on inhibiting bacterial growth
摘要
Abstract
Objective.The effect of juglone on S.aureaus and E cofi growth in different stages and morphological characteristics were studied, so as to discover the antibacterial mechanism of juglone. Method： The UV spectrophotometer and enumeration platecount method were used to determine cell growth curves.Observations of bacteria morphological characteristics were conducted by Transmission electron microscopy （TEX）. Result： The result showed that the growth of E.cofi in lag phase and log phase were obviously influenced by juglone.While for S.aureaus,its growth in log phase was more sensitive to juglone.Result from observation by TEX showed that the cytoplasm was concentrated and the cell was plasmolysed 2 hours after adding juglone to cultivative medium at minimum inhibition concentration（ MIC） .The cell wall was damaged seriously and the contents were leaked out after 8 hours.While for S.aureus,the cells absorbed water to expand,and part of the cell membrane was destroyed 4 hours after adding juglone to cultivative medium at MIC.Eight hours later, bacterial were adherent and boundaries tended to be illegible. Conclusion-It was suggested that juglone might influence bacterial growth at logarithmic stage.Also, it might inhibit growth of bacteria through damaging cell wall or cell membrane,关键词
胡桃醌/大肠杆菌/金黄色葡萄球菌/MIC/细菌形态Key words
juglone/Escherichia coli/Staphylococcus aureus/MIC/bacteria morphological分类
轻工纺织引用本文复制引用
吴朝霞,金楠,张敏,张旋..胡桃醌抑制细菌生长作用的研究[J].食品工业科技,2012,33(6):131-134,4.基金项目
辽宁省科学技术基金项目（20102195）. （）
