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嗜热棉毛菌圈体发酵产木聚糖酶条件的优化

范光森 杨绍青 闫巧娟 严烨 江正强

食品工业科技2012,Vol.33Issue(6):219-224,6.
食品工业科技2012,Vol.33Issue(6):219-224,6.

嗜热棉毛菌圈体发酵产木聚糖酶条件的优化

Optimization of xylanase production from Thermomyces lanuginosus CAU44 by solid-state fermentation

范光森 1杨绍青 1闫巧娟 2严烨 1江正强1

作者信息

  • 1. 中国农业大学食品科学与营养工程学院,北京100083
  • 2. 中国农业大学工学院,北京100083
  • 折叠

摘要

Abstract

The single-factor tests were employed to evaluate the optimal fermentation conditions for xylanase production of Thermomyces lanuginosus CAU44 by solid-state fermentation using agricultural wastes as carbon source.The results showed that the optimum conditions for xylanase production were as follows.mixture of corncob and wheat bran as carbon source (4:6), 1.5g/L( NH4 )2SO4 as nitrogen source, the ratio of solid substrate to mineral solution ratio was 1:4,the initial pH was 4.0, Tween-60 addition was 0.5%, and incubation temperature was 50%3. Under optimized conditions,the highest enzyme activity of 20343U/g dry carbon source was obtained at 7d, which was the highest value ever reported in domestic.Thus, xylanase production by Thermomyces lanuginosus CAU44 processed a potential application from the industrial point of view due to the high level of xylanase production and low producing cost.

关键词

嗜热棉毛菌/木聚糖酶/固体发酵/农业废弃物

Key words

Thermomyces lanuginosus/xylanase/solid- state fermentation/agricultural wastes

分类

轻工纺织

引用本文复制引用

范光森,杨绍青,闫巧娟,严烨,江正强..嗜热棉毛菌圈体发酵产木聚糖酶条件的优化[J].食品工业科技,2012,33(6):219-224,6.

基金项目

863计划课题 ()

新世纪优秀人才支持计划(NCET-08-0534). ()

食品工业科技

OA北大核心CSCDCSTPCD

1002-0306

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