食品工业科技2012,Vol.33Issue(6):219-224,6.
嗜热棉毛菌圈体发酵产木聚糖酶条件的优化
Optimization of xylanase production from Thermomyces lanuginosus CAU44 by solid-state fermentation
摘要
Abstract
The single-factor tests were employed to evaluate the optimal fermentation conditions for xylanase production of Thermomyces lanuginosus CAU44 by solid-state fermentation using agricultural wastes as carbon source.The results showed that the optimum conditions for xylanase production were as follows.mixture of corncob and wheat bran as carbon source (4:6), 1.5g/L( NH4 )2SO4 as nitrogen source, the ratio of solid substrate to mineral solution ratio was 1:4,the initial pH was 4.0, Tween-60 addition was 0.5%, and incubation temperature was 50%3. Under optimized conditions,the highest enzyme activity of 20343U/g dry carbon source was obtained at 7d, which was the highest value ever reported in domestic.Thus, xylanase production by Thermomyces lanuginosus CAU44 processed a potential application from the industrial point of view due to the high level of xylanase production and low producing cost.关键词
嗜热棉毛菌/木聚糖酶/固体发酵/农业废弃物Key words
Thermomyces lanuginosus/xylanase/solid- state fermentation/agricultural wastes分类
轻工纺织引用本文复制引用
范光森,杨绍青,闫巧娟,严烨,江正强..嗜热棉毛菌圈体发酵产木聚糖酶条件的优化[J].食品工业科技,2012,33(6):219-224,6.基金项目
863计划课题 ()
新世纪优秀人才支持计划(NCET-08-0534). ()