食品工业科技2012，Vol.33Issue(6)：272-275,279,5.
响应面法优化水酶法结合磷酸化提取大豆蛋白的生产工艺
Optimization of production process of enzyme-assisted aqueous extraction of soybean protein with phosphorylation by response surface method
摘要
Abstract
In order to improve the total extraction rate of soybean protein in the process of enzyme-assisted aqueous extraction,sodium tripolyphosphate（STP）was used to modify the protein in process of the enzymatic hydrolysis.The amount of the enzyme, liquid to material ratio, additive amount of STP, hydrolysis time and the influence of modification time on the total protein rate were studied.Meanwhile, response surface analysis method was employed to optimize experimental parameters.The total protein extraction rate was about 94.05% when the optimum parameters were chosen as follows：enzyme additive amount 5000U ~ g -~ substrate,material to liquid ratio 1：8,additive amount of STP 4%, hydrolysis time 3.37h and modification time 35.68min.关键词
磷酸化/化学改性/大豆/水酶法Key words
phosphorylation/chemical modification/soybeans/enzyme-assisted aqueous分类
轻工纺织引用本文复制引用
刘雯,江连洲,李杨,隋晓楠..响应面法优化水酶法结合磷酸化提取大豆蛋白的生产工艺[J].食品工业科技,2012,33(6):272-275,279,5.基金项目
黑龙江省攻关项目（GA091M01-6）. （）
