食品工业科技2012，Vol.33Issue(7)：108-111,4.
α-转移葡萄糖苷酶的转苷作用机理探究
Exploration into the enzymatic mechanism of α-transglucosidase
黄金莲 1黄秀娟 1钟振声1
作者信息
- 1. 华南理工大学化学与化工学院,广东广州510640
- 折叠
摘要
Abstract
Using High Performance Liquid Chromatography（HPLC）to track the transfer-glycoside process all the way.The enzymatic mechanism of α-glucosidase transferring maltose into isomaltose has been discussed.The results showed that in the transformation process,the α-glucosidase cut the α-1,4 glycosidic bonds into two glucose units firstly,then re-bonded by α-1,6 glycosidic linkage.And the isomerization process from maltose to isomaltose finished.The transglycosidation was happened intramolecularly.At the same time,the glucose units which dissociated from maltose reacted intermolecularly with maltose or isomaltose to generate panose or isomaltotriose.关键词
α-转移葡萄糖苷酶/异麦芽糖/催化/机理/转苷Key words
α-transglucosidase/isomaltose/catalysis/mechanism/transglycosidation分类
轻工纺织引用本文复制引用
黄金莲,黄秀娟,钟振声..α-转移葡萄糖苷酶的转苷作用机理探究[J].食品工业科技,2012,33(7):108-111,4.
