食品工业科技2012，Vol.33Issue(7)：181-183,187,4.
转谷氨酰胺酶酶原在大肠杆菌中的重组优化表达
Recombinant expression and optimization of pro-transglutaminase gene in Escherichia coli
摘要
Abstract
The pro-transglutaminase gene was subjected to codon usage optimization and then expressed in E.coli.Results demonstrated that GC content of the optimized gene decreased and its expression level was improved by 3.4-fold than the unoptimized gene.In order to improve the specific activity of transglutaminase,the serine at the second site of transglutaminase was mutated to proline by site-directed mutagenesis technology.The specific activity was 1.26-fold of the unmutated gene.The above results suggested that codon usage optimization and site-directed mutagenesis technology could be utilized to improve the expression level of pro-transglutaminase in E.coli.关键词
转谷氨酰胺酶酶原/密码子优化/定点突变/大肠杆菌/表达Key words
pro-transglutaminase(pro-TG)/codon usage optimization/site-directed mutagenesis/Escherichia coli/expression分类
生物科学引用本文复制引用
王坤,杨慧林,王斌,潘力..转谷氨酰胺酶酶原在大肠杆菌中的重组优化表达[J].食品工业科技,2012,33(7):181-183,187,4.基金项目
广东省科技攻关项目 （）
