食品工业科技2012，Vol.33Issue(8)：78-82,5.
荧光PCR快速检测A、C、G群溶血性链球菌
Rapid detection of group A, C and G Streptococcus hemolyticus by using real-time fluorescence PCR assays
摘要
Abstract
In order to beyond the limits that current real- time PCR method could only detect group A Streptococcus,a new method was established using real-time fluorescence PCR assays with detection range consistent to the national standard GB/T4789.11-2003. Bacitracin sensitive experiment was used to validate the detection range of Streptococcus hemolyticus in the national standard GB/T4789.11. Group A, C and G Streptococcus were selected and the DNA sequences of which were aligned using CLUSTAL X software. Conserved region of DNA sequences was used for the designing of primers and taqman probe.Method using real -time fluorescence PCR assays was constructed.The specificity, sensitivity and accuracy of this method were tested.Sensitivity experiment demonstrated that the new method could successfully distinguish the group A,C and G Streptococcus serotype from non-group A,C and G Streptococcus and other serotype.Simulated contamination experiment showed that after 18h culture at least 3,50 and 2CFU/mL of group A, C and G Streptococcus respectively could be tested by the new method from the enrichment broth. Accuracy test experiment was performed and completely same result was found either using the new method or national standard methods.This real-time fluorescence PCR assays could be used to rapid detection of Streptococcus hemolyticus in food, and might provide the reference for the national standards revision.关键词
实时荧光PCR/溶血性链球菌/检测/食品Key words
real-time PCR/Streptococcus hemolyticus/detection/food分类
轻工纺织引用本文复制引用
林霖,兰全学,祝仁发,杨国武..荧光PCR快速检测A、C、G群溶血性链球菌[J].食品工业科技,2012,33(8):78-82,5.基金项目
国家质检总局科技计划项目（2008QK272）. （）
