食品工业科技2012，Vol.33Issue(8)：95-98,116,5.
银杏类黄酮O-甲基转移酶活性的高效液相色谱分析
Analysis on flavonoid O-methyltransferase activity in Ginkgo by high performance liquid chromatography
摘要
Abstract
To research and get a technique and method for determination on the activity of fiavonoid O-methyltransferase（ FOMT）, a key-enzyme of flavonoid biosynthesis in Ginkgo leaves, by RP-HPLC for the first time.The adult leaves of male plants, were obtained from Ginkgo germplasm resources garden of Yangzhou university, then that were used to extract the crude enzyme solution. Based on the principle of S-adenosyl-L- homocysteine （SAH）derived from FOMT catalytic reaction and the correlationship between content and peak area of SAH,the relative activities of FOMT on the different substrates such as kaempferol, quercetin and isorhamnetin, were determined, the standard curve detected by optimized high performance liquid chromatography （HPLC） system in which there was a significant linear relationship within the range of 1.5-20μg/mL, RSD = 2.1% （n = 5）, so the optimized system for assaying FOMT activity had been established.FOMT activities of the different substrates showed different, kaempferol and isorhamnetin significiantly higher than quercetin. This HPLC system for determination on FOMT activity could be considered as a method which was accuracy, fastness, reliability and high sensitivity.关键词
银杏/雄株/叶片/类黄酮O-甲基转移酶（FOMT）活性/高效液相色谱Key words
Ginkgo/male plant/leaf/flavonoid/- methyltransferase (FOMT) activity/high performance liquid chromatography分类
轻工纺织引用本文复制引用
仲月明,张传丽,陈鹏,沈丹红,吴秋月,周长远..银杏类黄酮O-甲基转移酶活性的高效液相色谱分析[J].食品工业科技,2012,33(8):95-98,116,5.基金项目
基金项目：国家农业综合开发项目 （）
江苏省林业三项工程项目（1xsx[2008]02）. （）
