食品工业科技2012，Vol.33Issue(8)：137-139,142,4.
冷胁迫对冷冻干燥罗伊氏乳酸杆菌细胞膜流动性的影响
Effect of cold stress on membrane fluidity of Lactobacillus reuteri during freeze-drying
摘要
Abstract
The 1,6- diphenyl- 1,3,5- hexatriene （DPH） was used as fluorescence probe to label the membrane of Lactobacillus reuteri.The optimum conditions were established to measure the membrane fluidity of L.reuteri by labeling with DPH： excitation wavelength 360nm, emission wavelength 430nm, adjust ODs0o of L.reuteri to 0.8, incubateed at 30℃ in the dark for 20 min.Meanwhile,the survival rates,viability and membrane fluidity of L.reuten were measured after cold stress during freeze-drying.The results showed that the survival rates of freeze-drying Lactobacillus reuteri were improved by cold stress.At same time,the membrane fluidity was changed.The results implied that cold stress could improve cryotolerance of L.reuteri and prevent damage of freeze-drying.关键词
冷胁迫/罗伊氏乳酸杆菌/DPH/细胞膜流动性/冷冻干燥Key words
cold stress/Lactobacillus reuteri/DPH/membrane fluidity/freezedrying分类
轻工纺织引用本文复制引用
李宝坤,田丰伟,刘小鸣,赵建新,张灏,宋元达,陈卫..冷胁迫对冷冻干燥罗伊氏乳酸杆菌细胞膜流动性的影响[J].食品工业科技,2012,33(8):137-139,142,4.基金项目
国家自然基金 （）
“十一五”国家科技支撑计划重点项目 （）
“十二五”国家“863”计划（2011AA100901,2011AA100902）. （）
