食品工业科技2012，Vol.33Issue(8)：215-218,4.
响应面法分析优化米曲霉产β-葡萄糖苷酶液体发酵培养基
Optimization and analysis of β-glucosidase production by using response surface methodology
摘要
Abstract
Response surface analysis was conducted on Aspergillus oryzae ASPE0485 to determine the optimal culture medium for β-glucosidase fermentation.The Box-Behnken and Plackett-Burman design was utilized to the response surface analysis to determine the optimal culture medium consistents（ %, w/v）were as follow： corncob 3.8% ,soya peptone 0.5% ,KH2PO4 0.5% ,MgSO4 . 7H2O 0.05% ,CaCI2 0.05% ,tween-80 0.27% and inoculum size 5.3%.The β-glucosidase activity was 21.1U/mL under the optimal conditions, which was more 19.55% than the original strain activity（17.65U/mL）.关键词
米曲霉/β-葡萄糖苷酶/响应面分析法Key words
Aspergillus oryzae/β- glucosidase/response surface analysis分类
轻工纺织引用本文复制引用
朱婧,王青艳,刘演景,申乃坤,陈桂光,梁智群..响应面法分析优化米曲霉产β-葡萄糖苷酶液体发酵培养基[J].食品工业科技,2012,33(8):215-218,4.基金项目
桂科攻 （）
桂科攻（1099070）. （）
