食品工业科技2012，Vol.33Issue(9)：171-174,4.
羧基隐性孔雀石绿半抗原及全抗原的合成鉴定
Preparation and identification of hapten and complete antigens of carboxy-leucomalachite green
摘要
Abstract
Carboxy-leucomalachite green（COOH-LMG）was synthesized facilely with N,N-dimethylanilin and 4-Carboxybenzaldehyde as raw materials,and it was identified by the thin layer chromatography（TLC）,infrared spectrometry（IRS）,ultraviolet spectrum（UV）and liquid chromatography-mass spectrometer（LC-MS）.COOH-LMG was conjugated to bovine serum albumin（BSA）,ovalbumin（OVA）to form antigen and immunogen,and they were identified by UV spectra analysis.The antibodies against leucomalachite green（LMG）were obtained from rabbits after five times immunizations.The titer of the polyclonal antibody against the LMG was about 1：64000 by indirect ELISA.It laid the foundation for further gain monoclonal anti-LMG and its immunological assay.关键词
隐性孔雀石绿/合成/半抗原/人工抗原/多克隆抗体Key words
leucomalachite green/synthetize/hapten/complete antigens/polyclonal antibody分类
生物科学引用本文复制引用
张佳艳,伍金娥,常超..羧基隐性孔雀石绿半抗原及全抗原的合成鉴定[J].食品工业科技,2012,33(9):171-174,4.基金项目
湖北省自然科学基金 （）
武汉工业学院研究生创新基金 （）
