食品工业科技2012，Vol.33Issue(9)：194-196,201,4.
酶法合成共轭亚油酸单甘酯的研究
Study on enzymatic synthesis of monoacylglycerols enrich conjugated linoleic acid
摘要
Abstract
Monoacylglycerols enrich conjugated linoleic acid were produced using Penicillium lipase（G50）catalyzed glycerol with CLA.The esterification reaction was carried out in a solvent-free system.The effects of molar ratio of substrates,enzyme dosage,water content,reaction temperature and reaction time were evaluated.The results showed that the optimal reaction conditions were as follows：the mole ratio of glycerol to CLA 4：1,enzyme dosage 300U/g,water content in the system 1%,reaction temperature 15℃,reaction time 24h.Under the optimal conditions,the esterification rate was 84.98%,the MAG and DAG content in the finished product were 68.40% and 16.58% respectively.Through the analysis of the fatty acid composition of product,Penicillium lipase G50 was found no separation effect of CLA isomers.关键词
共轭亚油酸（CLA）/共轭亚油酸单甘酯（MAG）/酯化反应/脂肪酶Key words
CLA/MAG/esterification reaction/lipase分类
轻工纺织引用本文复制引用
高会场,徐达,孙丽娟,杨博,陈华勇,赵继伦..酶法合成共轭亚油酸单甘酯的研究[J].食品工业科技,2012,33(9):194-196,201,4.基金项目
广东省科技攻关 （）
