食品工业科技2012，Vol.33Issue(9)：205-208,4.
固定化脂肪酶Lipase G 50催化合成甘油二酯的研究
Study on immobilization of Lipase G 50 and its synthesis of diacylglycerol
摘要
Abstract
Lipase G 50 was adsorbed on 3 macroporous resin,including D380,CAT600 and AB-8,and the immobilized lipase was used to synthesize diacylglycerol by the esterification between glycerol and fatty acid.The maximum lipase activity was obtained by immobilization on resin D380.Optimal conditions were obtained as follows：glycerol/FA molar ratio of 5：1,7.5% of immobilized lipase amount（w/w）and 35℃.The conversion rate of fatty acid was 76.39% and the esterification product contained 43.89% diacylglycerol,and no triacylglycerol after 96h under those optimum conditions.In addition,immobilized lipase G 50 had good stability,and the conversion rate of fatty acid and the content of diacylglycerol reached 65.76% and 38.45% respectively after 5 batches of catalysis reaction under the same condition.关键词
偏甘油酯脂肪酶/固定化/甘油二酯/酯化反应Key words
Mono-and diacylglycerol lipase/immobilization/diacylglycerol/esterification分类
生物科学引用本文复制引用
徐扬,王卫飞,陈华勇,王永华,杨博..固定化脂肪酶Lipase G 50催化合成甘油二酯的研究[J].食品工业科技,2012,33(9):205-208,4.基金项目
广州市重大科技专项计划 （）
