食品科学2012，Vol.33Issue(3)：110-115,6.
壳聚糖固定瑞士乳杆菌蛋白酶的条件研究
Optimization of Process Conditions for Immobilization of Lactobacillus helveticus Proteinase with Chitosan
摘要
Abstract
Immobilized Lactobacillus helveticus proteinase was prepared using chitosan as carrier and glutaraldehyde as crosslinking agent by adsorption and cross-linking method. The effects of condensate, chitosan concentration, enzyme amount, crosslinking time, and glutaraldehyde concentration on immobilization efficiency ofLactobacillus helveticus proteinase as indicated by the activity of immobilized proteinase were explored based on single factor experiments. The optimal immobilization conditions were determined by response surface analysis to be a mixture of NaOH and methanol at a ratio of 3：1 as condensate at a dose of 4 g/100 mL, 2.89 g/100 mL ehitosan concentration, 2.95 mg enzyme amount, 1 h cross-linking time and 0.4 g/100 mL glutaraldehyde concentration.关键词
瑞士乳杆菌蛋白酶/固定化/酶活力Key words
Lactobacillus helveticus proteinase/immobilization/enzyme activity分类
轻工纺织引用本文复制引用
王翠翠,郭宇星,潘道东..壳聚糖固定瑞士乳杆菌蛋白酶的条件研究[J].食品科学,2012,33(3):110-115,6.基金项目
国家自然科学基金青年科学基金项目 （）
江苏省自然科学基金项目 （）
江苏省教育厅高校自然科学基金项目 （）
南京师范大学特聘教授、高层次人才科研启动基金项目 （）
