食品科学2012，Vol.33Issue(3)：151-156,6.
小黄鱼免疫肽制备条件的响应面优化
Optimization of Hydrolysis Conditions for Production of Immune Peptides from Small Yellow Croaker Using Response Surface Methodology
摘要
Abstract
Objective： To optimize process conditions for the hydrolysis of small yellow croaker by trypsin（PTN 6.0S） to prepare peptides with high immuno-activity.Methods： the proliferative effect（expressed as stimulation index,SI） of small yellow croaker hydrolysate on mouse spleen cells was assessed by the MTT assay.Based on the index,single-factor experiments followed by response surface analysis were carried out for the optimization of hydrolysis conditions.Results： The optimal hydrolysis conditions for preparing immune peptides were hydrolysis at 41.66 ℃ for 18.24 h at a substrate concentration of 6 g/100 mL and an enzyme-to-substrate mass ratio of 3.13‰.Under these conditions,the predicted SI of small yellow croaker hydrolysate was 0.387.When the temperature,time and substrate concentration were modified as 42 ℃,18.2 h and 3‰,the actual SI was 0.369± 0.003,which was slightly lower than the predicted value.Thus,appropriate process conditions were achieved.关键词
小黄鱼/免疫肽/响应曲面法/MTT法Key words
small yellow croaker/immune peptides/response surface methodology/MTT assay分类
轻工纺织引用本文复制引用
何佳易,徐鑫,刘国艳,蔡丽丽,魏晓蕊,满其琪,卢正竹..小黄鱼免疫肽制备条件的响应面优化[J].食品科学,2012,33(3):151-156,6.基金项目
江苏省科技型中小企业技术创新资金项目 （）
