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DiversiLab系统沙门氏菌分子分型评价

许龙岩 袁慕云 林文丽 张旺 焦红

食品科学2012,Vol.33Issue(3):203-206,4.
食品科学2012,Vol.33Issue(3):203-206,4.

DiversiLab系统沙门氏菌分子分型评价

Molecular Typing Evaluation of Salmonella in Diversilab System

许龙岩 1袁慕云 1林文丽 1张旺 1焦红1

作者信息

  • 1. 广东出入境检验检疫局技术中心食品实验室,广东广州510623
  • 折叠

摘要

Abstract

Objective: To evaluate the typing performance of DiversiLab system(DVL) for Salmonella.Method: A DVL system,which is based on rep-PCR,was used to type 44 Salmonella isolates from exported and imported foods and compared with pulsed-field gel electrophoresis(PFGE).The Salmonella isolates were typed by rep-PCR into 22 groups.The same serotypes of Salmonella isolates from different countries and foods were distributed in the same group with a high similarity.The PFGE results obtained for Salmonella isolates assigned to the same group using rep-PCR indicated a low similarity among all other isolates except No.2 and No.15.Conclusion: DVL shows better typing ability but lower resolution for Salmonella than PFGE.

关键词

沙门氏菌/DiversiLab系统/rep-PCR/PFGE/分子分型

Key words

Salmonella/DiversiLab system/rep-PCR/PFGE/molecular typing

分类

生物科学

引用本文复制引用

许龙岩,袁慕云,林文丽,张旺,焦红..DiversiLab系统沙门氏菌分子分型评价[J].食品科学,2012,33(3):203-206,4.

基金项目

广东省科技计划项目 ()

广东出入境检验检疫局科研项目 ()

食品科学

OA北大核心CSCDCSTPCD

1002-6630

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