食品科学2012，Vol.33Issue(3)：238-242,5.
珠母贝糖胺聚糖PG-Ⅱ诱导人宫颈癌HeLa细胞凋亡的研究
HeLa Cells Apoptosis Induced by Glycosaminoglycan Extracted from Pinctada martensi Dunker
摘要
Abstract
Objective： To investigate the apoptosis-inducing and proliferation-inhibitory effect glycosaminoglycan PG-II extracted from Pinctada martensi Dunker on HeLa cells. Methods： The growth-inhibitory effect was tested by MTT assay. Morphological observations on HeLa cells apoptosis were carried out under inverted microscope and inverted fluorescence microscope. The biochemical changes of HeLa cell DNA during apoptosis were observed by agarose gel electrophoresis. The percentage of apoptotic cells and cell cycle blockage were detected by flow cytometry. Results： PG- II could inhibit the proliferation of HeLa cells as determined by MTr assay. Morphological changes in apoptotic HeLa cells were observed under inverted microscope and inverted fluorescence microscope. The agarose gel electropherogram of HeLa cells treated with PG-II for 24 h displayed characteristic DNA ladders from apoptotic HeLa cells with molecular mass between 200 bp and 2000 bp. The results of flow cytometry indicated that PG- H could block the HeLa cell cycle at the G1 phase and that the largest blocking effect was achieved after treatment for 24 h at the dose of 100 mg/L with an apoptosis index of 42.6%. Conclusion： PG- II can induce apoptosis in HeLa cells and consequently inhibit their proliferation.关键词
珠母贝糖胺聚糖/人宫颈癌HeLa细胞/细胞凋亡Key words
glycosaminoglycan from Pinctada martensi Dunker/HeLa cell/apoptosis分类
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吴红棉,金晓石,范秀萍,胡雪琼,钟敏,雷晓凌..珠母贝糖胺聚糖PG-Ⅱ诱导人宫颈癌HeLa细胞凋亡的研究[J].食品科学,2012,33(3):238-242,5.基金项目
广东省教育厅自然科学研究项目 （）
