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正交试验法优化超声提取枣核总黄酮

张吉祥 欧来良

食品科学2012,Vol.33Issue(4):18-21,4.
食品科学2012,Vol.33Issue(4):18-21,4.

正交试验法优化超声提取枣核总黄酮

Optimization by Orthogonal Array Design of Ultrasonic Extraction of Total Flavonoids from Chinese Date Seeds

张吉祥 1欧来良2

作者信息

  • 1. 廊坊师范学院化学与材料科学学院,河北廊坊065000
  • 2. 南开大学生物活性材料教育部重点实验室,分子生物研究所,天津300071
  • 折叠

摘要

Abstract

Objective: The optimal conditions for ultrasonic extraction of total flavonoids from Chinese date seeds were investigated by orthogonal array design. Methods: The content of total flavonoids was determined spectrometrically using rutin as reference substance. The effects of ultrasonic power, extraction time, material/liquid ratio, ethanol concentration on the content of total flavonoids in extracts were investigated by one-factor-at-a-time method, and the extraction conditions were optimized by orthogonal array design. Results: Ethanol concentration and ultrasonic power were the key factors that affect the extraction of total flavonoids. The optimal extraction conditions were obtained as follows: ultrasonic power 450 W, treatment time 30 min, material/liquid ratio 1:50 and ethanol concentration 50%. Under these conditions, the extraction yield of crude total flavonoids from Chinese date seeds was 7.04% and the content of total flavonoids in crude extracts 1.59%. Conclusion: The optimized process remarkably increases the extraction yield of total flavonoids from Chinese date seeds and has a promising prospect for practical applications.

关键词

正交试验法/超声/提取/枣核/总黄酮

Key words

orthogonal array design/ultrasonic/extraction/Chinese date seeds/total flavonoids

分类

农业科技

引用本文复制引用

张吉祥,欧来良..正交试验法优化超声提取枣核总黄酮[J].食品科学,2012,33(4):18-21,4.

基金项目

河北省教育厅青年基金项目 ()

食品科学

OA北大核心CSCDCSTPCD

1002-6630

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