食品科学2012，Vol.33Issue(4)：144-147,4.
HPLC测定水产品中孔雀石绿、亚甲基蓝、结晶紫及其代谢物的残留量
Simultaneous Determination of Malachite Green, Methylene Blue, Crystal Violet as well as Their Metabolites in Aquatic Products by High Performance Liquid Chromatography
摘要
Abstract
A solid-phase extraction coupled with high performance liquid chromatography （SPE-HPLC） was presented for the simultaneous determination of malachite green, methylene blue, crystal violet as well as their metabolites （leucomalachite green for malachite green, azure A, azure B, azure C for methylene blue, and leucocrystal violet for crystal violet）. Samples were extracted with a mixture of ammonium acetate buffer and acetonitrile, cleaned up with dichioromethane, purified on an MCAX solid phase extraction cartridge using a mobile phased made up of acetonitrile and 0.125 mol/L ammonium acetate buffer, and detected by a UV detector. The limits of quantification （LOQ） of the method were 20 μg/kg for malachite green, leucomalachite green, crystal violet, leucocrystal violet and azure B and 0.5μg/kg for methylene blue, azure A and azure C, respectively. An average spike recovery above 70% was found for malachite green, leucomalachite green, leucocrystal violet, crystal violet and azure B at spike levels ranging from 2 to 40 μg/kg and for methylene blue, azure A and azure C at spike levels between 5 μg/kg and 80 μ g/kg, with a relative standard derivation （RSD） less than 15%.关键词
高效液相色谱/水产品/孔雀石绿/亚甲基蓝/结晶紫/代谢物Key words
high performance liquid chromatography/aquatic products/malachite green/methylene blue/crystalviolet~ metabolite分类
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宫向红,徐英江,任传博,刘慧慧,田秀慧,张世娟..HPLC测定水产品中孔雀石绿、亚甲基蓝、结晶紫及其代谢物的残留量[J].食品科学,2012,33(4):144-147,4.基金项目
国家海洋公益性行业科研专项 （）
山东省水生动物营养与饲料泰山学者岗位资助项目 （）
