食品科学2012，Vol.33Issue(4)：209-213,5.
高效液相色谱-串联质谱法测定动物肌肉组织中氨基甲酸酯类杀虫剂及其代谢物残留
Simultaneous Determination of Carbamate Insecticides and Metabolite Residues in Animal Muscle by High Performance Liquid Chromatography-Tandem Mass Spectrometry
邓龙 1郭新东 2何强 3杨群华 3沈玉栋 1王弘1
作者信息
- 1. 广东省食品质量安全重点实验室,华南农业大学食品质量与安全研究所,广东广州510642
- 2. 广州市质量监督检测研究院,广东广州510110
- 3. 广东省农药检定所,广东广州510500
- 折叠
摘要
Abstract
A high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry （HPLC- ESI/MS/MS） method for rapid determination of 16 residual analytes including carbamate insecticides and their metabolites in animal muscles was established. Samples were extracted with acetonitrile, and the extract was then condensed, cleaned up, analyzed by reversed phase HPLC-ESI/MS/MS, and quantified by internal standard method. The calibration curves for the analytes exhibited a good linear relationship in the range of 1.0- 100 μg/L with correlation coefficient higher than 0.9959. The limits of quantification （LOQ） ranged from 0.5 to 2.5 μg/kg. The recovery rates of the analytes from animal muscle sample across the spike levels of 5.0, 10.0 μ g/kg and 20 μg/kg were in the range of 71.4% - 105.5% with relative standard deviation （RSD） varying from 3.2% to 13.7%. The method was accurate, sensitive, simple and suitable for the simultaneous analysis of carbamate insecticides and their metabolite residues in animal muscle.关键词
氨基甲酸酯/液相色谱串联质谱/动物肌肉组织Key words
carbamate/high performance liquid chromatography coupled with electrospray ionization tandem massspectrometry (HPLC-ESI/MS/MS)/animal muscle分类
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邓龙,郭新东,何强,杨群华,沈玉栋,王弘..高效液相色谱-串联质谱法测定动物肌肉组织中氨基甲酸酯类杀虫剂及其代谢物残留[J].食品科学,2012,33(4):209-213,5.
