食品科学2012，Vol.33Issue(4)：234-237,4.
高效液相色谱-原子荧光法测定水产品中甲基汞含量
Determination of Methylmercury in Aquatic Products by HPLC-AFS
摘要
Abstract
A high performance liquid chromatography-atomic fluorescence spectrometry （HPLC-AFS） method was developed for the determination of methylmercury in aquatic products. Samples were extracted with a mixture of vitriol, potassium chloride and hydrochloric acid. Then the extract was cleaned up on an SPE column, diluted with the HPLC mobile phase and filtrated through a 0.45 tam pore size membrane prior to HPLC-AFS analysis. The quantification of methylmercury was carried out using the external standard method. Good linearity between chromatographic peak area and methylmercury concentration ranging from 2 to 50 μ g/L was found （R^2 =0.9995）. Average recoveries for methylmercury in different blank aquatic products spiked at 20, 200,1000 μ g/kg were 80.2% - 87.1% with relative standard deviations （RSD） between 3.4% and 6.8%. The method exhibited a detection limit of 20μg/kg. The method had the advantages of simplicity and little matrix interference, and meanwhile its precision and accuracy could meet the requirements for the analysis of methylmercury in aquatic products.关键词
甲基汞/水产品/高效液相色谱-原子荧光法Key words
methylmercury/aquatic product/HPLC-AFS分类
农业科技引用本文复制引用
陈雪昌,梅光明,张小军,朱敬萍,郭远明,顾捷,郑斌..高效液相色谱-原子荧光法测定水产品中甲基汞含量[J].食品科学,2012,33(4):234-237,4.基金项目
2009年农业部行业标准制定项目 （）
