食品科学2012，Vol.33Issue(5)：66-70,5.
Zn^2＋对球等鞭金藻3011细胞膜电位和膜通透性的影响
Effect of Zn^2＋ on Membrane and Membrane Permeability of Isochrysis galbana 3011
摘要
Abstract
Flow cytometry and fluorescence microscope were used to explore the effect of Zn^2＋ on membrane potential and membance permeability in lsochrysis galbana 3011. The processed microalga were detected by flow cytometry and fluorescence microscope with DiBAC4（3） and PI staining through single-factor tests. The statistical software of SPSS 17.0 was used for variance analysis of the data. The results showed that the membrane potential in macrophage was increased significantly and the membance permeability was changed after stimulated with Zn^2＋ in 5 μ g/L for 4 min. Therefore, a dynamic membrane status was observed and 5 μ g/L Zn^2＋ could lead to partial membrane depolarization and change the membance permeability of lsochrysis galbana 3011, which may be linked to sodium ion channel.关键词
球等鞭金藻3011/锌离子/流式细胞技术/膜电位/膜通透性Key words
Isochrysis galbana 3011/Zn^2+/flow cytometry/membrane potential/membrane permeability分类
农业科技引用本文复制引用
赵培,王雪青,陈庆森,彭博丽,亢凯,陈欢..Zn^2＋对球等鞭金藻3011细胞膜电位和膜通透性的影响[J].食品科学,2012,33(5):66-70,5.基金项目
天津市自然科学基金重点项目 （）
