食品科学2012，Vol.33Issue(5)：118-123,6.
包埋-交联磷脂酶A1聚集体的制备及酶学性质
Preparation of Encapsulation-crosslinked Aggregates of Phospholipase A1
摘要
Abstract
In order to prepare reusable immobilized phospholipase A1 with high activity, the encapsulation-crosslinked aggregate method was used. The immobilization conditions and partial properties of immobilized phospholipase were determined. Results indicated that the optimal preparation conditions were enzyme concentration of 0.06 g/mL, precipitant saturation degree of 80%, precipitation time of 35 min, glutaradehyde concentration of 0.4%, cross-linking time of 6.5 h, sodium alginate concentration of 0.2% and Ca^2＋ concentration of 0.25 mol/L. Under the optimal preparation conditions, the recovery rate of enzyme activity was 80.2%. The thermal stability of immobilized phospholipase was better than that of free phospholipase. Meanwhile, after seventh repeated use, the relative activity of immobilized phospholipase still remained more than 65%.关键词
磷脂酶A1/沉淀剂/戊二醛/交联酶聚集体/固定化/包埋/扫描电镜（SEM）Key words
phospholipase A1/precipitan: glutaraldehyde: cross-linked enzyme aggregates (CLEAs):immobilization/encapsulation: SEM分类
生物科学引用本文复制引用
王妍,王雪,李越,李志平,于殿宇..包埋-交联磷脂酶A1聚集体的制备及酶学性质[J].食品科学,2012,33(5):118-123,6.基金项目
“十二五”国家科技支撑计划项目 （）
困家“863”计划项目 （）
