食品科学2012，Vol.33Issue(5)：134-137,4.
有机溶剂对多聚半乳糖醛酸酶催化动力学的影响
Effect of Organic Solvent on Catalytic Kinetics of Polygalacturonase
刘小林 1李晓婷 2龚国勇 2黄磊 3黄卓烈 4伍志权 4黎春怡4
作者信息
- 1. 宜春学院生命科学学院,江西宜春336000／华南农业大学生命科学学院,广东广州510642
- 2. 宜春学院生命科学学院,江西宜春336000
- 3. 江西宜春中学,江西宜春336000
- 4. 华南农业大学生命科学学院,广东广州510642
- 折叠
摘要
Abstract
Water-soluble chitosan with low molecular weight was employed to modify polygalacturonase （PG）. After modification, the catalytic kinetics of PG and modified polygalacturonase （COS-PG） were investigated in buffer, methanol, ethanol, acetone and tetrahydrofumn solution using pectin as substrate. The results indicated that Km of COS-PG revealed a slight decline, but Vmax revealed an incarease. Km and Vmax of PG and COS-PG declined both in 2% methanol and 2% ethanol. Km and Vmax of PG and COS-PG increased in 2% tetrahydrofuran solution. Conversely, in 2% acetone solution, Km increased but Vmax declined.关键词
多聚半乳糖醛酸酶/有机溶剂/动力学Key words
polygalacturonase/organic solvents/dynamics分类
生物科学引用本文复制引用
刘小林,李晓婷,龚国勇,黄磊,黄卓烈,伍志权,黎春怡..有机溶剂对多聚半乳糖醛酸酶催化动力学的影响[J].食品科学,2012,33(5):134-137,4.
