食品科学2012，Vol.33Issue(5)：150-154,5.
米根霉高效利用玉米淀粉生产乳酸的发酵条件优化
Optimization of Fermentation Conditions for Efficient Production of Lactic Acid from Corn Starch by Rhizopus oryzae
摘要
Abstract
The fermentation conditions of lactic acid produced from corn starch by Rhizopus oryzae were investigated. Rhizopus oryzae HB 12 was newly isolated from soil samples. Based on single factor and orthogonal tests, the optimal fermenttation medium was composed of 140 g/L corn starch, 2 g/L NH4C1, 0.3 g/L KH2PO4, 0.3 g/L MgSO4· 7H2O, 0.05 g/L ZnSO4·7H2O and 80 g/L CaCO3. The optimal fermentation conditions were 2.5 ×10^6 spores in 50 mL of medium cultured at 35 ℃, 200 r/min shaking for 108 h in 250 mL flask. Under the optimal fermentation conditions, the yield of lactic acid produced from corn starch was up to 104.9 g/L with a conversion of 74.9%. The volumetric production rate was up to 0.97 g/（L· h）, which revealed 49.4% enhancement compared with the original conditions. Therefore, Rhizopus oryzae HB12 can effectively utilize inexpensive corn starch for the production of lactic acid, which will have good prospect for industrial application.关键词
米根霉/乳酸/发酵条件/玉米淀粉Key words
Rhizopus oryzae/lactic acid/fermentation condition/corn starch分类
化学化工引用本文复制引用
徐忠义,闫巧娟,江正强,蔡威,王新磊..米根霉高效利用玉米淀粉生产乳酸的发酵条件优化[J].食品科学,2012,33(5):150-154,5.基金项目
国家“973”计划项目 （）
