食品科学2012，Vol.33Issue(5)：177-182,6.
浓香型白酒窖泥中高产己酸兼性厌氧细菌的分离鉴定
Isolation and Identification of Facultative Anaerobic Strains with High Yield of Hexanoic Acid from Luzhou-flavor Liquor Pit Mud
摘要
Abstract
In order to screen and identify hexanoic acid-producing anaerobic bacteria with high yield from liquor pit mud and to apply it in maintaining pits or artificial pit mud. The hexanoic acid-producing anaerobic strains with high yield were isolated and selected from the fine pit mud of the distillery in the northeast area by facultative anaerobic culture and trace component analysis. The yields ofhexanoic acid were up to 213.6914, 170.465 mg/100 mL and 103.5097 mg/100 mL, respectively. The species identification showed that three strains were Bacillusfusiformis, Bacillus licheniformis and Bacillus megaterium, respectively, through morphological observation, physiological and biochemical tests and molecular biology methods. The optimal pH was 7, 6.5 and 7 and the optimal temperatures were 34, 34 ℃ and 37 ℃ as well as the optimal inoculums were 5%, 5% and 3%. This study provides a theatrical guidance for the application of efficiently artificial pit mud.关键词
浓香型白酒/窖泥/产己酸细菌/鉴定Key words
Luzhou-flavor liquor/pit mud/hexanoic acid-producing bacteria/identification分类
生物科学引用本文复制引用
赵辉,敞颜,王葳,凌宏志,平文祥,赵志昌,杨朝辉..浓香型白酒窖泥中高产己酸兼性厌氧细菌的分离鉴定[J].食品科学,2012,33(5):177-182,6.基金项目
哈尔滨市科技局科技创新人才专项基金项目（2011RFQXN056）：黑龙江省教育厅科学技术研究项目 （）
